Breathitt Veterinary Center Histopathology Department

The Histopathology department specializes in the preparation of normal and diseased tissues for microscopic evaluation by a pathologist. Techniques utilized in this section include tussue fixation, processing, microtomy, routine and special stains.

The preservation of tissues in 10% neutral buffered formalin stabilizes tissue proteins and celular structure. During processing, the tissues undergo dehydration through gradient alcohols, clearing of the dehydrant agents, and infiltration with paraffin (wax). Paraffin holds the tissue, both cells and intercellular structures intact, allowing for uniform consistency during microtomy. We perform routine staining of 4 micron thick tissue sections with hematoxylin and eosin (H & E); this method demonstrates the nucleus, cytoplasm, muscle, and connective tissue. We use special stains to confirm abnormal growth, bacterial or fungal infections. A pathologist interprets and reports the results.